Polymerase chain reaction and restriction fragment length polymorphism , pcr - rflp 限制性片段長度多態(tài)性
1983 restriction fragment length polymorphism ( rflp ) technique used in cancer research 1983年,限制片段長度多態(tài)( rflp )技術(shù)在癌癥研究中應(yīng)用
Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell 小鼠限制性片段長度多態(tài)性dna指紋圖jl - 02探針胚胎干細(xì)胞
1 . because the taxonomic division is rather complex and has been much disputed and revised , in this part , we will review the classification and phylogeny of families , subfamilies and tribes of anseriformes based on morphology , ethology , osteology , mitochondrial and nuclear dna restriction fragment length polymorphism , single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition , classification and phylogenetic relationships of the families , subfamilies and tribes of anseriformes . the controversial questions and deficiency in the systematic studies of anseriformes were pointed out 具體包括以下幾個部分: 1 、針對雁形目鳥類異常復(fù)雜的分類狀況及分類上存在的爭議,根據(jù)雁形目鳥類的形態(tài)學(xué)、行為學(xué)、骨骼學(xué)、角蛋白、線粒體與核dna酶切片段長度多態(tài)、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究,對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統(tǒng)發(fā)生關(guān)系進(jìn)行綜述,分析系統(tǒng)學(xué)研究中存在的不足,提出了雁形目鳥類分類中急需解決的問題。
All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc ) 所有的樣本通過聚合酶鏈?zhǔn)椒磻?yīng)?限制性片段長度多態(tài)性方法( pcr - rflp )對ahsg基因的第7個外顯子內(nèi)的sac多態(tài)性位點(diǎn)進(jìn)行基因分型,該多態(tài)性位點(diǎn)為238號氨基酸密碼子中間的堿基c到g的替換,使蘇氨酸( thr , acc )變?yōu)榻z氨酸( ser , agc ) 。
The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates 本研究通過對豬繁殖與呼吸綜合征病毒( prrsv )國內(nèi)分離毒株的gp3 、 gp5和n蛋白的抗原性比較及其orf5和orf7遺傳變異性分析,系統(tǒng)研究了國內(nèi)分離毒株的抗原特性和遺傳學(xué)差異。
Analyse the research of distributing of microbe community and the tendency of the change , disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism , to research the law of change that the microbe community have in composting process , we can get effective and rapid information to filtrate the microorganism during composting process , then accelerate the development of compost technology 摘要對堆肥微生物種群分布及其動態(tài)變化的研究進(jìn)行了分析,論述了分子生物技術(shù)中的變性梯度凝膠電泳和末端標(biāo)記限制性片段長度多態(tài)性的原理和特點(diǎn),以及用于研究堆肥微生物的群落結(jié)構(gòu)演變規(guī)律,為分析和篩選堆肥中的微生物提供更加有效、快速的信息,促進(jìn)堆肥技術(shù)的發(fā)展。
Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province . 2 . the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough 應(yīng)用熔解曲線法和pcr微板核酸雜交- elisa法對44例接受拉米夫定治療過程中出現(xiàn)病毒學(xué)反跳時的血清進(jìn)行ymdd突變株的檢測,并與測序法和mpcr - rflp法比較它們的敏感性、一致性。
In molecular biology, restriction fragment length polymorphism, or RFLP (commonly pronounced “rif-lip”), is a technique that exploits variations in homologous DNA sequences. It refers to a difference between samples of homologous DNA molecules that come from differing locations of restriction enzyme sites, and to a related laboratory technique by which these segments can be illustrated.